Presentation Title

Programmed Cell Death in a Chick Model of Diabetic Embryopathy.

University

Shawnee State University

Major

Biology (Biomedical Science)

Student Type

Undergraduate Student

Presentation Types

Oral Group Presentation

Keywords:

Diabetes, Birth Defects, Apoptosis

Description

Diabetes mellitus in the mother is the one of the most prominent instigators of birth defects. Here we continue an investigation into the effects of increased glucose environment on the developing Gallus gallus domesticus embryo. A 30mM glucose solution was injected into the yolk of Hamburger and Hamilton stage 6 (HH6) embryos. Controls included saline injected and un-injected eggs. At ~HH14 and ~HH18 stages embryos from all treatment groups were assessed for gross malformation. Neural tube deformities and cardiac defects were most frequently observed. We hypothesize that programmed cell death plays a role in the malformations. Embryos from all treatment groups were assayed for apoptotic cells using an in situ cell death detection kit. An expansion of the pattern and intensity of stained apoptotic cells was observed in embryos treated with 30mM glucose compared to controls. Future studies will explore the timing of apoptosis relative to glucose injection.

Faculty Sponsor Name

Kimberly Inman

Faculty Sponsor Title

Associate Professor of Biology

Faculty Sponsor Academic Department

Natural Sciences

Location

KRI 150

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Mar 28th, 10:00 AM Mar 28th, 10:50 AM

Programmed Cell Death in a Chick Model of Diabetic Embryopathy.

KRI 150

Diabetes mellitus in the mother is the one of the most prominent instigators of birth defects. Here we continue an investigation into the effects of increased glucose environment on the developing Gallus gallus domesticus embryo. A 30mM glucose solution was injected into the yolk of Hamburger and Hamilton stage 6 (HH6) embryos. Controls included saline injected and un-injected eggs. At ~HH14 and ~HH18 stages embryos from all treatment groups were assessed for gross malformation. Neural tube deformities and cardiac defects were most frequently observed. We hypothesize that programmed cell death plays a role in the malformations. Embryos from all treatment groups were assayed for apoptotic cells using an in situ cell death detection kit. An expansion of the pattern and intensity of stained apoptotic cells was observed in embryos treated with 30mM glucose compared to controls. Future studies will explore the timing of apoptosis relative to glucose injection.