University
Shawnee State University
Major
Premed Biology
Presentation Types
Oral Presentation (Live)
Keywords:
gelatinase, zymography, zymogram
Abstract
Streptococcus pyogenes has been shown to produce an extracellular cysteine protease which degrades gelatin, fibronectin, collagen, and other extracellular matrix (ECM) proteins. Degradation of the ECM allows bacteria to more easily invade tissues, which may lead to increased virulence. The production of gelatin-degrading enzymes was examined in Escherichia coli and Streptococcus pyogenes using zymography. By incorporation of gelatin into polyacrylamide gels, zymograms can determine the presence of active enzymes that degrade gelatin. This information may be useful in exploring the role of gelatinases in bacterial pathogenicity.
Human and Animal Subjects
no
IRB or IACUC Approval
no
Faculty Mentor Name
Eugene Burns
Faculty Mentor Title
Professor of Biology
Faculty Mentor Department
Natural Sciences
Recommended Citation
Barnhart, Allen, "Determination of Bacterial Gelatinase Production By Zymography" (2021). Celebration of Scholarship. 8.
https://digitalcommons.shawnee.edu/cos/2021/day2/8
Determination of Bacterial Gelatinase Production By Zymography
Streptococcus pyogenes has been shown to produce an extracellular cysteine protease which degrades gelatin, fibronectin, collagen, and other extracellular matrix (ECM) proteins. Degradation of the ECM allows bacteria to more easily invade tissues, which may lead to increased virulence. The production of gelatin-degrading enzymes was examined in Escherichia coli and Streptococcus pyogenes using zymography. By incorporation of gelatin into polyacrylamide gels, zymograms can determine the presence of active enzymes that degrade gelatin. This information may be useful in exploring the role of gelatinases in bacterial pathogenicity.
