Tuesday, 4/5/2022
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University
Shawnee State University
Major
Biology - Biomedical Sciences
Presentation Types
Oral Group Presentation
Keywords:
AML, Cancer, Warburg Effect
Abstract
Acute Myeloid Leukemia (AML) is one of many cancer types that exhibit the Warburg Effect. This effect is characterized by cells utilizing glycolysis for ATP production even when oxygen is present. Dichloroacetate inhibits aerobic glycolysis by activating pyruvate dehydrogenase, forcing the cell to go through oxidative phosphorylation, and allowing mitochondrial functions in apoptosis to be restored. The AML cell lines, HL-60 and THP-1, were used as a model system in this study. After kill curve analysis, a dosage dependent decrease in cell counts were seen after 48 hours of treatment. To investigate the mechanism of this effect, proteins involved with cell cycle regulation and apoptosis will be examined. Cdk2 is a protein involved in cell cycle machinery, and mTOR regulates cell metabolism. Expression levels of these proteins will be measured to provide a basis for a potential mechanism of action and prospective novel treatments for this deadly disease.
Human Subjects
no
IRB Approval
no
Faculty Mentor Name
Dr. Jennifer Napper
Faculty Mentor Title
Associate Professor of Biology
Faculty Mentor Academic Department
Natural Sciences
Recommended Citation
Tackett, Hanna and Lacy, Ali, "The Warburg Effect in Acute Myeloid Leukemia" (2022). Celebration of Scholarship. 7.
https://digitalcommons.shawnee.edu/cos/2022/day2/7
The Warburg Effect in Acute Myeloid Leukemia
Acute Myeloid Leukemia (AML) is one of many cancer types that exhibit the Warburg Effect. This effect is characterized by cells utilizing glycolysis for ATP production even when oxygen is present. Dichloroacetate inhibits aerobic glycolysis by activating pyruvate dehydrogenase, forcing the cell to go through oxidative phosphorylation, and allowing mitochondrial functions in apoptosis to be restored. The AML cell lines, HL-60 and THP-1, were used as a model system in this study. After kill curve analysis, a dosage dependent decrease in cell counts were seen after 48 hours of treatment. To investigate the mechanism of this effect, proteins involved with cell cycle regulation and apoptosis will be examined. Cdk2 is a protein involved in cell cycle machinery, and mTOR regulates cell metabolism. Expression levels of these proteins will be measured to provide a basis for a potential mechanism of action and prospective novel treatments for this deadly disease.