Thursday, 4/7/2022
University
Shawnee State University
Major
Biology
Presentation Types
Oral Presentation (Live)
Keywords:
Cancer MGT AML EGCG
Abstract
Acute myeloid leukemia (AML) is a deadly cancer of the bone marrow and blood. Cancerous myeloid cells can travel from the bone marrow into the bloodstream, causing overcrowding of the remaining functional cells. We have found that when AML cells are treated with matcha green tea (MGT), cell proliferation decreases. In THP-1 cells, the knockdown of circadian rhythm genes Bmal1 and CLOCK both lead to a decrease in cell viability. One of the polyphenols in MGT, epigallocatechin-3-gallate, has been linked to circadian rhythm by inhibiting the circadian protein CLOCK. This indicates that epigallocatechin-3-gallate might also target other circadian rhythm proteins that function within the same pathway, such as Bmal1. The aim of this study is to investigate the possible mechanisms of Bmal1, CLOCK, and EGCG in THP-1 cell death. The results from this study could provide a novel therapy needed to treat this deadly disease.
Human and Animal Subjects
no
Faculty Mentor Name
Jennifer Napper
Faculty Mentor Title
Professor of Biology
Faculty Mentor Department
Natural Sciences
Recommended Citation
Long, Megan, "The Effects of Epigallocatechin-3-gallate and Circadian Rhythm Genes in THP-1 Acute Myeloid Leukemia Cells" (2022). Celebration of Scholarship. 10.
https://digitalcommons.shawnee.edu/cos/2022/day4/10
The Effects of Epigallocatechin-3-gallate and Circadian Rhythm Genes in THP-1 Acute Myeloid Leukemia Cells
Acute myeloid leukemia (AML) is a deadly cancer of the bone marrow and blood. Cancerous myeloid cells can travel from the bone marrow into the bloodstream, causing overcrowding of the remaining functional cells. We have found that when AML cells are treated with matcha green tea (MGT), cell proliferation decreases. In THP-1 cells, the knockdown of circadian rhythm genes Bmal1 and CLOCK both lead to a decrease in cell viability. One of the polyphenols in MGT, epigallocatechin-3-gallate, has been linked to circadian rhythm by inhibiting the circadian protein CLOCK. This indicates that epigallocatechin-3-gallate might also target other circadian rhythm proteins that function within the same pathway, such as Bmal1. The aim of this study is to investigate the possible mechanisms of Bmal1, CLOCK, and EGCG in THP-1 cell death. The results from this study could provide a novel therapy needed to treat this deadly disease.
