Bordetella bronchiseptica and Pasteurella multocida Attachment to Pig Cells

Marli HoldernessFollow

University

Shawnee State University

Major

Biomedical Sciences

Student Type

Undergraduate Student

Presentation Types

Oral Presentation

Keywords:

B. bronchiseptica, P. multocida, attachment, PTK cells

Abstract

Respiratory infections such as atrophic rhinitis are a source of substantial economic loss to pig farmers. Often, both Bordetella bronchiseptica and Pasteurella multocida are isolated from the respiratory tract of infected animals and both may contribute to disease. This study is designed to explore whether the presence of one of these pathogens affects attachment of the other bacteria to epithelial cells. Porcine nasal turbinate epithelial cells (PTK-75 cells) were grown in tissue culture and exposed to B. bronchiseptica and P. multocida separately. The attachment of each bacteria was observed microscopically and the number of bacteria attached per 50 pig cells was determined. To see if secreted proteins produced by one of the bacteria affect attachment by the other, culture supernatant from one bacteria will be added into the attachment assay with the other bacteria. Similarly, whole cell lysates from one bacteria will be added to attachment assays using the other bacteria to determine if non-secreted proteins have an effect on attachment. Comparison of attachment levels between the bacteria alone and the bacteria with supernatant or lysate from the other bacteria will reveal whether the attachment of either of these organisms is affected by the other.

Human Subjects

no

Faculty Mentor Name

Eugene Burns

Faculty Mentor Title

Professor

Faculty Mentor Academic Department

Natural Sciences

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Bordetella bronchiseptica and Pasteurella multocida Attachment to Pig Cells

Respiratory infections such as atrophic rhinitis are a source of substantial economic loss to pig farmers. Often, both Bordetella bronchiseptica and Pasteurella multocida are isolated from the respiratory tract of infected animals and both may contribute to disease. This study is designed to explore whether the presence of one of these pathogens affects attachment of the other bacteria to epithelial cells. Porcine nasal turbinate epithelial cells (PTK-75 cells) were grown in tissue culture and exposed to B. bronchiseptica and P. multocida separately. The attachment of each bacteria was observed microscopically and the number of bacteria attached per 50 pig cells was determined. To see if secreted proteins produced by one of the bacteria affect attachment by the other, culture supernatant from one bacteria will be added into the attachment assay with the other bacteria. Similarly, whole cell lysates from one bacteria will be added to attachment assays using the other bacteria to determine if non-secreted proteins have an effect on attachment. Comparison of attachment levels between the bacteria alone and the bacteria with supernatant or lysate from the other bacteria will reveal whether the attachment of either of these organisms is affected by the other.