Interactions between Bordetella bronchiseptica and Pasteurella multocida in Attachment to Porcine Epithelial Cells
University
Shawnee State University
Major
Biology
Student Type
Undergraduate Student
Presentation Types
Oral Presentation (Live)
Keywords:
B. bronchiseptica, P. multocida, attachment, PTK cells
Abstract
Respiratory infections such as atrophic rhinitis are a source of substantial economic loss to pig farmers. Often, both Bordetella bronchiseptica and Pasteurella multocida are isolated from the respiratory tract of infected animals and both may contribute to disease. This study is designed to explore whether the presence of one of these pathogens affects attachment of the other bacteria to epithelial cells. Porcine nasal turbinate epithelial cells (PTK-75 cells) were grown in tissue culture and exposed to B. bronchiseptica and P. multocida separately. Attachment of each bacteria was determined using standard plate counts. To see if secreted proteins produced by one of the bacteria affect attachment by the other, filtered culture supernatant from one bacteria was added into the attachment assay with the other bacteria. Initial experiments suggest that secreted proteins of Pasteurella multocida increase the attachment of Bordetella bronchiseptica but further experimentation will be needed to determine significance. Similarly, whole cell lysates from one bacteria will be added to attachment assays using the other bacteria to determine if non-secreted proteins have an effect on attachment.
Human and Animal Subjects
no
IRB or IACUC Approval
no
Faculty Mentor Name
Eugene Burns
Faculty Mentor Title
Professor
Faculty Mentor Department
Natural Sciences
Recommended Citation
Holderness, Marli, "Interactions between Bordetella bronchiseptica and Pasteurella multocida in Attachment to Porcine Epithelial Cells" (2025). Celebration of Scholarship. 3.
https://digitalcommons.shawnee.edu/cos/2025/session2/3
Location
LIB 204
Interactions between Bordetella bronchiseptica and Pasteurella multocida in Attachment to Porcine Epithelial Cells
LIB 204
Respiratory infections such as atrophic rhinitis are a source of substantial economic loss to pig farmers. Often, both Bordetella bronchiseptica and Pasteurella multocida are isolated from the respiratory tract of infected animals and both may contribute to disease. This study is designed to explore whether the presence of one of these pathogens affects attachment of the other bacteria to epithelial cells. Porcine nasal turbinate epithelial cells (PTK-75 cells) were grown in tissue culture and exposed to B. bronchiseptica and P. multocida separately. Attachment of each bacteria was determined using standard plate counts. To see if secreted proteins produced by one of the bacteria affect attachment by the other, filtered culture supernatant from one bacteria was added into the attachment assay with the other bacteria. Initial experiments suggest that secreted proteins of Pasteurella multocida increase the attachment of Bordetella bronchiseptica but further experimentation will be needed to determine significance. Similarly, whole cell lysates from one bacteria will be added to attachment assays using the other bacteria to determine if non-secreted proteins have an effect on attachment.
