Quantification of Caffeine via GC-MS

Riddoc WebbFollow

University

Shawnee State University

Major

Biology and Chemistry

Student Type

Undergraduate Student

Presentation Types

Oral Presentation (Live)

Keywords:

Caffeine, GC-MS, SIM

Abstract

Soda and energy drinks share one key common factor, caffeine. An addictive stimulant used for allowing people to stay awake for longer periods of time, when lacking rest. While the quantification of caffeine is normally done via UV-Vis spectroscopy, a different approach was used. This different approach was the use of a GC-MS, via mixing 25 μL of soda or energy drink and 1 mL of chloroform. Using the selected ion mode, to easily and quickly determine the concentration of caffeine in each sample, a time of 90 seconds was found to be optimal for finding the maximum signal. This allows the samples to be separated and analyzed with a high level of accuracy and precision.

Human and Animal Subjects

no

Faculty Mentor Name

Andrew Napper

Faculty Mentor Title

Professor of Chemistry

Faculty Mentor Department

Natural Sciences

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Quantification of Caffeine via GC-MS

Soda and energy drinks share one key common factor, caffeine. An addictive stimulant used for allowing people to stay awake for longer periods of time, when lacking rest. While the quantification of caffeine is normally done via UV-Vis spectroscopy, a different approach was used. This different approach was the use of a GC-MS, via mixing 25 μL of soda or energy drink and 1 mL of chloroform. Using the selected ion mode, to easily and quickly determine the concentration of caffeine in each sample, a time of 90 seconds was found to be optimal for finding the maximum signal. This allows the samples to be separated and analyzed with a high level of accuracy and precision.