Programmed Cell Death in a Chick Model of Diabetic Embryopathy.
University
Shawnee State University
Major
Biology (Biomedical Science)
Presentation Types
Oral Group Presentation
Keywords:
Diabetes, Birth Defects, Apoptosis
Abstract
Diabetes mellitus in the mother is the one of the most prominent instigators of birth defects. Here we continue an investigation into the effects of increased glucose environment on the developing Gallus gallus domesticus embryo. A 30mM glucose solution was injected into the yolk of Hamburger and Hamilton stage 6 (HH6) embryos. Controls included saline injected and un-injected eggs. At ~HH14 and ~HH18 stages embryos from all treatment groups were assessed for gross malformation. Neural tube deformities and cardiac defects were most frequently observed. We hypothesize that programmed cell death plays a role in the malformations. Embryos from all treatment groups were assayed for apoptotic cells using an in situ cell death detection kit. An expansion of the pattern and intensity of stained apoptotic cells was observed in embryos treated with 30mM glucose compared to controls. Future studies will explore the timing of apoptosis relative to glucose injection.
Faculty Mentor Name
Kimberly Inman
Faculty Mentor Title
Associate Professor of Biology
Faculty Mentor Academic Department
Natural Sciences
Recommended Citation
Marsh, Caleb; Stewart, Jessie; and Conley, Alexis, "Programmed Cell Death in a Chick Model of Diabetic Embryopathy." (2019). Celebration of Scholarship. 6.
https://digitalcommons.shawnee.edu/cos/2019/day2/6
Location
KRI 150
Programmed Cell Death in a Chick Model of Diabetic Embryopathy.
KRI 150
Diabetes mellitus in the mother is the one of the most prominent instigators of birth defects. Here we continue an investigation into the effects of increased glucose environment on the developing Gallus gallus domesticus embryo. A 30mM glucose solution was injected into the yolk of Hamburger and Hamilton stage 6 (HH6) embryos. Controls included saline injected and un-injected eggs. At ~HH14 and ~HH18 stages embryos from all treatment groups were assessed for gross malformation. Neural tube deformities and cardiac defects were most frequently observed. We hypothesize that programmed cell death plays a role in the malformations. Embryos from all treatment groups were assayed for apoptotic cells using an in situ cell death detection kit. An expansion of the pattern and intensity of stained apoptotic cells was observed in embryos treated with 30mM glucose compared to controls. Future studies will explore the timing of apoptosis relative to glucose injection.