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University
Shawnee State University
Major
Biomedical Sciences
Presentation Types
Oral Group Presentation
Keywords:
diabetes, chicken embryos, glucose
Abstract
Diabetes mellitus is a serious disease that affects 60 million women of reproductive age worldwide, a number that is expected to double by 2030. In pregnancy, the disease is associated with a nine-fold increase in birth defects, heightening concern about its rising prevalence. Previous research in our lab has identified birth defects in chick embryos within 48 hours of introduction to a diabetic environment. The goal of this project is to determine the glucose content of both control and glucose-treated embryos. To achieve this, we will adapt a commercially available enzymatic glucose oxidase assay originally designed for measurement of glucose in food and subsequently adapted for use in Drosophila, manure, and cell culture. In this project, fertilized chicken eggs were injected with known concentrations of glucose and embryos were collected following incubation. Serial dilutions of a glucose standard and embryo homogenates were assayed and absorbance measured to determine glucose content.
Human Subjects
no
Faculty Mentor Name
Kimberly Inman
Faculty Mentor Title
Associate Professor and Chair of the Department of Natural Sciences
Faculty Mentor Academic Department
Natural Sciences
Recommended Citation
Spencer, Mallory and Cruse, Jacob, "Adapting a Commercially Available Glucose Assay for Use with Chick Embryos" (2021). Celebration of Scholarship. 7.
https://digitalcommons.shawnee.edu/cos/2021/day4/7
Adapting a Commercially Available Glucose Assay for Use with Chick Embryos
Diabetes mellitus is a serious disease that affects 60 million women of reproductive age worldwide, a number that is expected to double by 2030. In pregnancy, the disease is associated with a nine-fold increase in birth defects, heightening concern about its rising prevalence. Previous research in our lab has identified birth defects in chick embryos within 48 hours of introduction to a diabetic environment. The goal of this project is to determine the glucose content of both control and glucose-treated embryos. To achieve this, we will adapt a commercially available enzymatic glucose oxidase assay originally designed for measurement of glucose in food and subsequently adapted for use in Drosophila, manure, and cell culture. In this project, fertilized chicken eggs were injected with known concentrations of glucose and embryos were collected following incubation. Serial dilutions of a glucose standard and embryo homogenates were assayed and absorbance measured to determine glucose content.