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University
Shawnee State University
Major
Biomedical Sciences / Pre-Med
Presentation Types
Oral Presentation
Keywords:
metformin, AML, leukemia, cancer
Abstract
Acute myeloid leukemia (AML) is a highly deadly cancer which is characterized by an over-proliferation of immature white blood cells, leading to crowding out of functional cells. Our research focuses on the effects that metformin, a drug used to treat type II diabetes, has on AML cells. The primary hypothesis being tested is that metformin will decrease the survivability of these leukemia cells. To that end, two lines of AML cells are being used as a model system to test our hypothesis, THP-1 and HL-60. We have demonstrated that metformin kills these cells in a dosage dependent manner. To determine a mechanism of action, we plan to examine proteins using western blotting. Two target proteins that we will measure expression of are Cdk2, which is a protein that is involved in the cell cycle, and Caspase-3, a protein that is involved in apoptosis.
Human Subjects
no
IRB Approval
no
Faculty Mentor Name
Dr. Jennifer Napper
Faculty Mentor Title
Associate Professor of Biology
Faculty Mentor Academic Department
Natural Sciences
Recommended Citation
Kielmar, Jacob F. and Phipps, Aaron C., "Effect of Metformin on Acute Myeloid Leukemia Cells" (2021). Celebration of Scholarship. 3.
https://digitalcommons.shawnee.edu/cos/2021/day5/3
Effect of Metformin on Acute Myeloid Leukemia Cells
Acute myeloid leukemia (AML) is a highly deadly cancer which is characterized by an over-proliferation of immature white blood cells, leading to crowding out of functional cells. Our research focuses on the effects that metformin, a drug used to treat type II diabetes, has on AML cells. The primary hypothesis being tested is that metformin will decrease the survivability of these leukemia cells. To that end, two lines of AML cells are being used as a model system to test our hypothesis, THP-1 and HL-60. We have demonstrated that metformin kills these cells in a dosage dependent manner. To determine a mechanism of action, we plan to examine proteins using western blotting. Two target proteins that we will measure expression of are Cdk2, which is a protein that is involved in the cell cycle, and Caspase-3, a protein that is involved in apoptosis.