Friday, 4/8/2022
University
Shawnee State University
Major
Biology/Biomedical Sciences
Presentation Types
Oral Presentation (Live)
Keywords:
metformin, AML, leukemia, cancer
Abstract
Acute myeloid leukemia (AML) is a highly deadly cancer which is characterized by an over-proliferation of immature white blood cells, leading to crowding out of functional cells. My research focuses on the effects that metformin, a drug used to treat type II diabetes, has on AML cells. The primary hypothesis being tested is that metformin will decrease the survivability of these leukemia cells. To that end, THP-1 cells, an AML cell line, was used as a model system. I have demonstrated that metformin kills these cells in a dosage dependent manner. To determine a mechanism of this toxicity, we measured protein expression using western blotting. The target protein I examined this semester was cdk2.
Human and Animal Subjects
no
IRB or IACUC Approval
no
Faculty Mentor Name
Dr. Jennifer Napper
Faculty Mentor Title
Associate Professor of Biology
Faculty Mentor Department
Natural Sciences
Recommended Citation
Kielmar, Jacob, "Metformin Treatment of Acute Myeloid Leukemia Cells" (2022). Celebration of Scholarship. 5.
https://digitalcommons.shawnee.edu/cos/2022/day5/5
Metformin Treatment of Acute Myeloid Leukemia Cells
Acute myeloid leukemia (AML) is a highly deadly cancer which is characterized by an over-proliferation of immature white blood cells, leading to crowding out of functional cells. My research focuses on the effects that metformin, a drug used to treat type II diabetes, has on AML cells. The primary hypothesis being tested is that metformin will decrease the survivability of these leukemia cells. To that end, THP-1 cells, an AML cell line, was used as a model system. I have demonstrated that metformin kills these cells in a dosage dependent manner. To determine a mechanism of this toxicity, we measured protein expression using western blotting. The target protein I examined this semester was cdk2.
