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University
Shawnee State University
Major
Biology - Biomedical Sciences
Presentation Types
Event
Keywords:
Cancer, TNBC, AML, Warburg Effect
Abstract
Acute Myeloid Leukemia (AML) and Triple Negative Breast Cancer (TNBC) are two of many cancer types that exhibit the Warburg Effect. This effect is characterized by cells utilizing glycolysis for ATP production even when oxygen is present. Dichloroacetate inhibits aerobic glycolysis by activating pyruvate dehydrogenase, forcing the cell to go through oxidative phosphorylation, which allows mitochondrial functions in apoptosis to be restored. HL-60 cells, an AML cell line, and BT-20 cells, a TNBC cell line, were used as model systems in this study. Treatment dosage was determined by treating the cells with various concentrations of dichloroacetate. To investigate the mechanism of toxicity, a protein involved with cell cycle regulation will be examined. CDC20 is involved in mitotic spindle assembly. Expression levels of the protein will be measured with and without treatment to provide a basis for a potential mechanism of action and prospective novel treatments for these deadly diseases.
Human Subjects
no
Faculty Mentor Name
Dr. Jennifer Napper
Faculty Mentor Title
Chairperson and Professor of Biology
Faculty Mentor Academic Department
Natural Sciences
Recommended Citation
Tackett, Hanna, "The Warburg Effect in Acute Myeloid Leukemia and Triple Negative Breast Cancer" (2023). Celebration of Scholarship. 13.
https://digitalcommons.shawnee.edu/cos/2023/Day4/13
The Warburg Effect in Acute Myeloid Leukemia and Triple Negative Breast Cancer
Acute Myeloid Leukemia (AML) and Triple Negative Breast Cancer (TNBC) are two of many cancer types that exhibit the Warburg Effect. This effect is characterized by cells utilizing glycolysis for ATP production even when oxygen is present. Dichloroacetate inhibits aerobic glycolysis by activating pyruvate dehydrogenase, forcing the cell to go through oxidative phosphorylation, which allows mitochondrial functions in apoptosis to be restored. HL-60 cells, an AML cell line, and BT-20 cells, a TNBC cell line, were used as model systems in this study. Treatment dosage was determined by treating the cells with various concentrations of dichloroacetate. To investigate the mechanism of toxicity, a protein involved with cell cycle regulation will be examined. CDC20 is involved in mitotic spindle assembly. Expression levels of the protein will be measured with and without treatment to provide a basis for a potential mechanism of action and prospective novel treatments for these deadly diseases.